The identification of evolutionarily constrained sequences is an unbiased approach for finding functional sequences in genomes. However, their identification is strongly affected by upstream analyses.

Correspondence to Dr Parco M Siu, The University of Hong Kong, Hong Kong, 999077, Hong Kong; pmsiu{at}hku.hk Data sources Embase, MEDLINE, PsycINFO, Cochrane Library, Web of Science, Scopus and ...