Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a ...

Murine leukemia virus (MLV)-based vectors are still the most frequently used gene delivery vehicles, employed in almost 40% of approved clinical protocols worldwide. 1,2 Despite their frequent use as ...

In vivo protein expression includes cloning a gene of interest with the essential control elements into an expression vector, and then transforming the vector into an easily grown organism or cell ...

A universal vector (IL-60 and auxiliary constructs), expressing or silencing genes in every plant tested to date, is described. Plants that have been successfully manipulated by the IL-60 system ...

Recombinant protein expression is an essential pillar of biomedical research. Efforts from enzyme discovery to protein structure-function study to development of drugs and biological therapeutics all ...

Escherichia coli is widely considered the most cost-effective bacterial system for recombinant protein expression. Consequently, it is broadly used. Nonetheless, it has its limitations as an ...