When a ray of light that is travelling through a piece of glass strikes the interface between the glass and the air, it changes direction according to Snell’s law. If the angle of incidence is less ...
Such a thick layer can be observed when the liquid is held within a transparent container and the observer is looking at the top most portion of the liquid from the sides of the transparent container.
The DeltaVision OMX V4 enables Total Internal Reflection Fluorescence Microscopy (TIRF). This technique uses an evanescent wave to selectively excite fluorophores that are close to the coverslip. This ...