Western Blot 是分子生物学中研究蛋白的经典方法，被广泛应用于检测细胞或组织样本中蛋白表达量。传统的Western Blot被认为是定性或半定量Western Blot，那么高分文献中的定量Western Blot（Quantitative Western Blot）是如何实现的？小编和您一起来了解定量Western Blot。

无论是定量、定性或半定量，这都是相对的，因为没有绝对的测量。关键是要最大限度地提高准确性和精确度，使相对尽可能有意义。那如何尽可能减少可变性呢？此篇文章主要论述从Western Blot方法学和成像仪器的选择上来减少可变性。 Western Blot是定量 or 定性？

Western or immunoblotting analysis of protein expression in cells and tissues has been the major analytical tool for assessing molecular biological functions in basic cell biological research, ...

A western blot, sometimes called a protein immunoblot, is an antibody-based technique used to detect the presence, size and abundance of specific proteins within a sample. The technique was developed ...

The goal of western blotting is to reliably detect and quantify protein expression levels. When performed well, they can be accurate and cost-effective. But, according to a Bio-Rad survey of North ...

The western blot may appear to be just another staple lab technique. However, there are some stories surrounding its invention that are definitely worth knowing. This might be news to some: scientists ...